Shotgun metabolomic analysis of killer whale () exhaled breath condensate.

The ocean is facing many anthropogenic stressors caused from both pollution and climate change. These stressors are significantly impacting and changing the ocean's ecosystem, and as such, methods must continually be developed that can improve our ability to monitor the health of marine life. For cetaceans, the current practice for health assessments of individuals requires live capture and release, which is expensive, usually stressful, and for larger species impractical.

Proteomic profiles of Leptospira borgpetersenii serovar Hardjo strains JB197 and HB203 cultured at different temperatures.

Leptospirosis is a global zoonotic disease affecting humans, domestic, and wild animals. Leptospira are typically shed in the urine of reservoir hosts which persist in suitable environments where incidental host transmission occurs after direct contact with infected urine or contaminated environments. Interestingly, serologically identical L.

Draft genome sequence of a multidrug-resistant strain isolated from a dairy cow with chronic mastitis.

is considered an environmental pathogen, and it can cause acute and chronic mastitis in dairy cows. Here, we report the draft genome sequence of a multidrug-resistant strain (2011C-S1) isolated from a Holstein cow showing signs of chronic mastitis that was nonresponsive to intramammary antibiotic treatment.

Effect of selection genotype on immune response to Brucella abortus RB51 in Holstein cattle.

Genetic selection for milk production traits in US Holsteins has affected numerous genes associated with reproduction and immunity. This study compares the transcriptomic response of peripheral blood mononuclear cells to an in vitro Brucella abortus strain RB51 (RB51) bacterial challenge between contemporary Holsteins and Holsteins that have not been selected for milk production traits since the mid-1960s. Total RNA was extracted from peripheral blood mononuclear cells from four contemporary and four unselected lactating, primiparous cows following 24-h incubation with or without stimulation with RB51 bacteria.

Genome sequence of a multidrug-resistant strain isolated from a dairy cow that was nonresponsive to antibiotic treatment.

We report the draft genome sequence of a multidrug-resistant strain isolated from a Holstein cow with chronic mastitis. The assembled genome contained 108 contigs with an of 130,886 bp, 66.03% GC content, 6,214 protein-coding genes, 64 RNA genes, 88 pseudogenes, and six antibiotic-resistant genes.