Publications by authors named "J Lightley"

'openFrame' is a modular, low-cost, open-hardware microscopy platform that can be configured or adapted to most light microscopy techniques and is easily upgradeable or expandable to multiple modalities. The ability to freely mix and interchange both open-source and proprietary hardware components or software enables low-cost, yet research-grade instruments to be assembled and maintained. It also enables rapid prototyping of advanced or novel microscope systems.

View Article and Find Full Text PDF
Article Synopsis
  • A new, cost-effective method of differential phase microscopy has been developed using a special camera that captures four images at once.
  • This technique allows for the creation of phase contrast images, which help visualize samples more clearly.
  • It can also be easily combined with fluorescence microscopy for enhanced imaging capabilities.
View Article and Find Full Text PDF

We presenta robust, long-range optical autofocus system for microscopy utilizing machine learning. This can be useful for experiments with long image data acquisition times that may be impacted by defocusing resulting from drift of components, for example due to changes in temperature or mechanical drift. It is also useful for automated slide scanning or multiwell plate imaging where the sample(s) to be imaged may not be in the same horizontal plane throughout the image data acquisition.

View Article and Find Full Text PDF

Electron microscopy (EM) following immunofluorescence (IF) imaging is a vital tool for the diagnosis of human glomerular diseases, but the implementation of EM is limited to specialised institutions and it is not available in many countries. Recent progress in fluorescence microscopy now enables conventional widefield fluorescence microscopes to be adapted at modest cost to provide resolution below 50 nm in biological specimens. We show that stochastically switched single-molecule localisation microscopy can be applied to clinical histological sections stained with standard IF techniques and that such super-resolved IF may provide an alternative means to resolve ultrastructure to aid the diagnosis of kidney disease where EM is not available.

View Article and Find Full Text PDF

The case of a mildly handicapped man who inhaled a drawing pin is described. Early chest radiology is recommended in unexplained respiratory symptoms in mental handicap.

View Article and Find Full Text PDF