Fluorescence Detection of DNA/RNA G-Quadruplexes (G4s) by Twice-as-Smart Ligands.

Fluorescence detection of DNA and RNA G-quadruplexes (G4s) is a very efficient strategy to assess not only the existence and prevalence of cellular G4s but also their relevance as targets for therapeutic interventions. Among the fluorophores used to this end, turn-on probes are the most interesting since their fluorescence is triggered only upon interaction with their G4 targets, which ensures a high sensitivity and selectivity of detection. We reported on a series of twice-as-smart G4 probes, which are both smart G4 ligands (whose structure is reorganized upon interaction with G4s) and smart fluorescent probes (whose fluorescence is turned on upon interaction with G4s).

Drug-like Inhibitors of DC-SIGN Based on a Quinolone Scaffold.

DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin) is a pattern recognition receptor expressed on immune cells and involved in the recognition of carbohydrate signatures present on various pathogens, including HIV, Ebola, and SARS-CoV-2. Therefore, developing inhibitors blocking the carbohydrate-binding site of DC-SIGN could generate a valuable tool to investigate the role of this receptor in several infectious diseases. Herein, we performed a fragment-based ligand design using 4-quinolone as a scaffold.

Vacuum-formed retainers more effective than Hawley retainers.

Design: A randomised controlled trial (RCT) was performed in a single orthodontic practice.

Intervention: The aim was to compare the clinical effectiveness of Hawley and vacuum-formed retainers (VFR) over a 6-month period of retention. Three hundred and ninety-seven patients were treated by a specialist orthodontist and randomly allocated to wear either Hawley retainers (n=196) or VFR (n=201).

[Pediatric head lice: taxonomy, incidence, resistance, delousing].

Based on the current knowledge, head louse (Pediculus capitis) and body louse (Pediculus humanus) are two different species that infest specific parts of the human body and do not interbreed in vivo. In 1991-2002, 6 257 cases of pediculosis were reported in the Czech Republic while 3 138 000 pediculicide packagings, i.e.