Automated Nanoliter Volume Assay Optimization on a Cost-Effective Microfluidic Disc.

Optimizing multireagent assays often requires successive titration of individual components until the optimal combination of conditions is achieved. This process is time-consuming, laborious, and often expensive since parallelized experimentation requires bulk consumption of reagents. Microfluidics presents a solution through miniaturization of standard processes by reducing reaction volume, executing multiple parallel workflows, and enabling automation.

Using trauma video review to search for the Goldilocks pre-activation time.

Objectives: We sought to determine the optimal time to pre-activation for trauma team activation that resulted in maximum team efficiency, measured by the time to complete critical actions (TCCAs) during resuscitation. We hypothesized that there exists a time window for trauma team pre-activation that minimizes TCCA.

Methods: This is an exploratory retrospective analysis of video-reviewed traumas at a level 1 trauma center from January 1, 2018 to 28 February, 2022 that received the highest trauma team activation and had a pre-arrival notification.

Sex-specific DNA methylation differences in Amyotrophic lateral sclerosis.

Sex is an important covariate in all genetic and epigenetic research due to its role in the incidence, progression and outcome of many phenotypic characteristics and human diseases. Amyotrophic lateral sclerosis (ALS) is a motor neuron disease with a sex bias towards higher incidence in males. Here, we report for the first time a blood-based epigenome-wide association study meta-analysis in 9274 individuals after stringent quality control (5529 males and 3975 females).

Impact of coating type on structure and magnetic properties of biocompatible iron oxide nanoparticles: insights into cluster organization and oxidation stability.

Superparamagnetic iron oxide nanoparticles (SPIONs) are a promising tool for biomedical applications, including drug delivery, imaging, and magnetic hyperthermia. However, their tendency to agglomerate limits their performance efficiency. To overcome this limitation, a coating can be applied during or after synthesis.