Lymphocyte killer activity in individuals sensitized against antigen-D assessed by direct ADCC lysis of O,Rh-positive erythrocytes.

Lymphocytes from individuals sensitized against the Rh-D antigen were found to lyse O,Rh-positive erythrocytes. The controls consisted of individuals non-sensitized against D antigen. The lytic effect in the absence of additional in vitro added specific antibody was very high in sensitized individuals, but low in the control group.

In vitro investigation of Rh antibodies in pregnant women.

Seventeen Rh negative pregnant women who had Rhesus alloantibodies were investigated serologically. The antibody-mediated cellular cytotoxicity (ADCC) against target Rhesus positive human erythrocytes was determined. The investigation was repeated in the first, second and third trimester of pregnancy and after delivery.