Publications by authors named "J L Workman"
Objectives: To identify clinical presentations that acted as harbingers for future sepsis hospitalizations in pediatric patients evaluated in the emergency department (ED) using the Symptom Disease Pair Analysis of Diagnostic Error (SPADE) methodology.
Methods: We identified patients in the Pediatric Health Information Systems (PHIS) database admitted for sepsis between January 1, 2004 and December 31, 2023 and limited the study cohort to those patients who had an ED treat-and-release visit in the 30 days prior to admission. Using the look-back approach of the SPADE methodology, we identified the most common clinical presentations at the initial ED visit and used an observed to expected (O:E) analysis to determine which presentations were overrepresented.
Article Synopsis
- The Linearbandkeramik (LBK) Neolithic communities were pioneers in spreading agriculture across Europe and this study showcases genetic data from 250 individuals to understand their ancestry.
- The findings reveal a notable difference in ancestry, with eastern LBK sites having a higher percentage of western hunter-gatherer genetics compared to western sites, indicating separate genetic paths for these groups.
- Additionally, the research suggests a patrilocal social structure, featuring more genetic connections among males within sites, and points out that the massacre at Asparn-Schletz likely involved individuals from a large population rather than a small community.
Delivering ribonucleoproteins (RNPs) for in vivo genome editing is safer than using viruses encoding for Cas9 and its respective guide RNA. However, transient RNP activity does not typically lead to optimal editing outcomes. Here we show that the efficiency of delivering RNPs can be enhanced by cell-penetrating peptides (covalently fused to the protein or as excipients) and that lipid nanoparticles (LNPs) encapsulating RNPs can be optimized for enhanced RNP stability, delivery efficiency and editing potency.
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- The increase in Fragile X disorders (FXD) and immune-related issues indicates that factors beyond genetic mutations are involved in the disease's development.
- New findings show that altered expression and splicing of transposon element (TE)-derived genes that affect purine metabolism and immune responses to viruses are present in FXD patients.
- These TE-derived genes have a higher proportion of specific dinucleotides, resembling some RNA viruses, suggesting that previous viral infections may have influenced the immune disorders seen in FXD.
Serial capture affinity purification (SCAP) is a powerful method to isolate a specific protein complex. When combined with cross-linking mass spectrometry and computational approaches, one can build an integrated structural model of the isolated complex. Here, we applied SCAP to dissect a subpopulation of WDR76 in complex with SPIN1, a histone reader that recognizes trimethylated histone H3 lysine4 (H3K4me3).
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