Rat strains differ in antibody response to natural Haemophilus species infection.

Antibody response to Haemophilus species in rat strains was monitored by enzyme-linked immunosorbent assay (ELISA) using antigens of two Haemophilus strains and a Pasteurella pneumotropica strain. Five rat strains from a breeding colony naturally infected by Haemophilus were significantly different in ELISA antibody activity and in the number of seropositive animals. BN and RP rats were (relatively) high and low responders, respectively and BUF, LEW and WAG rats were intermediate.

Transmission of rat and guineapig Haemophilus spp. to mice and rats.

Mice and rats, free from Pasteurellaceae, were exposed to Haemophilus spp. (V-factor dependent Pasteurellaceae) by housing in proximity to infected rats or guinea pigs, and monitored by culture and enzyme-linked immunosorbent assay (ELISA) for cross infection. A minority of mice became infected when exposed to Haemophilus-infected rats but none when exposed to guinea pigs.

Serological relationship of some V-factor dependent Pasteurellaceae (Haemophilus sp.) from guineapigs and rabbits.

Culture of guinea pig and rabbit respiratory tracts for bacteria using X- (haemin) and V- (NAD) factor in agar media detected infection by V-factor dependent Pasteurellaceae (Haemophilus sp.) in three colonies of guinea pigs and a group of rabbits. The 12 Haemophilus strains comprised three API NH codes classed as Haemophilus parainfluenzae and two codes classed as Haemophilus aphrophilus/paraphrophilus.

An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Pasteurella pneumotropica antibodies.

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Pasteurella pneumotropica antibodies in the sera of rats, mice, hamsters and Mastomys. P. pneumotropica from mice and rats showed cross-reactivity.

Serological studies of Corynebacterium kutscheri and coryneform bacteria using an enzyme-linked immunosorbent assay (ELISA).

An enzyme-linked immunosorbent assay (ELISA) to measure Corynebacterium kutscheri antibodies in mice and rats was developed. Seven C. kutscheri isolates showed considerable serological relationship, but Japanese isolates differed from the British isolates.