Publications by authors named "J L Sherley"
Article Synopsis
- Developing advanced cell therapy products involves defining key concepts like mechanism of action (MOA), potency, and efficacy, which can be challenging but is crucial for success.
- The text introduces a logical framework that aligns with regulatory guidelines and draws on insights from 27 FDA-approved cell therapy products to better understand these concepts.
- It highlights the complexity of correlating potency test results with clinical outcomes, discusses common misconceptions, and emphasizes the need for effective communication among developers throughout the product development process.
Article Synopsis
- - Kinetic stem cell (KSC) counting is a new technology for accurately measuring tissue stem cells in various organ and tissue samples, addressing a significant gap in stem cell research.
- - The study tested KSC counting on human oral alveolar bone cell preparations, showing high precision in determining the fractions of mesenchymal stem cells (MSCs) across multiple independent sites.
- - The results also demonstrated KSC counting's capability to identify factors affecting different stem cell subtypes, supporting its potential to enhance advancements in stem cell science and related medical fields.
A continuing limitation and major challenge in the development and utilization of predictable stem cell therapies (SCTs) is the determination of the optimal dosages of stem cells. Herein, we report the quantification of stem cell fractions (SCF) of human mesenchymal stem cell (MSC) preparations derived from oral tissues. A novel computational methodology, kinetic stem cell (KSC) counting, was used to quantify the SCF and specific cell culture kinetics of stem cells in oral alveolar bone-derived MSC (aBMSCs) from eight patients.
View Article and Find Full Text PDFA recently described kinetic stem cell (KSC) counting method was used to investigate the stem-cell-specific effects of commercial growth factor supplements used for expanding stem cells in adipose-tissue-derived mesenchymal cell preparations. The supplements were a proprietary growth factor product, a source of fetal bovine serum, two sources of pooled human sera, and two sources of human platelet lysate. KSC counting analyses were performed to monitor effects on the fraction and viability of stem cells in serial cultures with their respective supplements.
View Article and Find Full Text PDFSince the U.S. Supreme Court issued its landmark decision in 1973 to legalize abortion, over 60 million preborn have been killed by elective abortion.
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