Filamin A C-terminal fragment modulates Orai1 expression by inhibition of protein degradation.

Filamin A (FLNA) is an actin-binding protein that has been reported to interact with STIM1 modulating the activation of Orai1 channels. Cleaving of FLNA by calpain leads to a C-terminal fragment that is involved in a variety of functional and pathological events, including pro-oncogenic activity in different types of cancer. Here we show that full-length FLNA is downregulated in samples from colon cancer patients as well as in the adenocarcinoma cell line HT-29.

Examining Interventions for Pediatric Adverse Childhood Experiences in an Integrated Primary Care Setting.

Introduction: Adverse Childhood Experiences (ACEs) increase the risk of chronic diseases in adulthood and contribute to neurobehavioral challenges during childhood and adolescence.

Method: This retrospective study reviewed the medical records of 102 pediatric patients to describe interventions for children screening positive for ACEs in a pediatric primary care setting with integrated behavioral health services. It further assessed whether these interventions led to improvements in psychosocial functioning and mood.

Planning for Long-Term Recovery: The Impact of COVID-19 on Educators Working With Children From Seasonal and Migrant Farmworker Families.

Background: Education faced challenges during the COVID-19 pandemic. Although the United States has declared an end to the public health emergency, schools continue to be impacted. While considerable research has focused on the impact of the pandemic on students, fewer studies have focused on educators, and even less on educators who specialize in working with marginalized student populations.

Feedback modulation of Orai1α and Orai1β protein content mediated by STIM proteins.

Store-operated Ca entry is a mechanism controlled by the filling state of the intracellular Ca stores, predominantly the endoplasmic reticulum (ER), where ER-resident proteins STIM1 and STIM2 orchestrate the activation of Orai channels in the plasma membrane, and Orai1 playing a predominant role. Two forms of Orai1, Orai1α and Orai1β, have been identified, which arises the question whether they are equally regulated by STIM proteins. We demonstrate that STIM1 preferentially activates Orai1α over STIM2, yet both STIM proteins similarly activate Orai1β.