Molecular Epidemiology of Oropouche Virus, Ceará State, Brazil, 2024.

During May-December 2024, we detected Oropouche virus (OROV) in 13.9% (263/1,890) of febrile patients in Ceará state, Brazil. Genomic sequencing revealed those cases were caused by a novel OROV reassortant previously identified in the Amazon region.

Using Machine Learning and Optical Microscopy Image Analysis of Immunosensors Made on Plasmonic Substrates: Application to Detect the SARS-CoV-2 Virus.

In this article, we introduce a diagnostic platform comprising an optical microscopy image analysis system coupled with machine learning. Its efficacy is demonstrated in detecting SARS-CoV-2 virus particles at concentrations as low as 1 PFU (plaque-forming unit) per milliliter by processing images from an immunosensor on a plasmonic substrate. This high performance was achieved by classifying images with the support vector machine (SVM) algorithm and the MobileNetV3_small convolutional neural network (CNN) model, which attained an accuracy of 91.

Diacerein reduces inflammasome activation and SARS-CoV-2 virus replication: a proof-of-concept translational study.

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is linked to high mortality, primarily through an intense inflammatory response. Diacerein has emerged as a potential therapy for COVID-19 due to its potential impact in decreasing the inflammasome activation and coronavirus replication. This study aims to explore diacerein's influence in inhibiting both viral replication and the inflammatory response after SARS-CoV-2 infection.