Evolutionary genomics of Leishmania braziliensis across the neotropical realm.

The Neotropical realm, one of the most biodiverse regions on Earth, houses a broad range of zoonoses that pose serious public health threats. Protozoan parasites of the Leishmania (Viannia) braziliensis clade cause zoonotic leishmaniasis in Latin America with clinical symptoms ranging from simple cutaneous to destructive, disfiguring mucosal lesions. We present the first comprehensive genome-wide continental study including 257 cultivated isolates representing most of the geographical distribution of this major human pathogen.

Methodological approaches on synergies and trade-offs within the 2030 Agenda.

The world is lagging in achieving the 2030 Agenda's 17 Sustainable Development Goals (SDGs), emphasizing social, economic, and environmental dimensions. Meeting one target may either help or hinder meeting another, but the high number of potential interactions complicates the evaluation of synergies and trade-offs. Here, we focus on the water-energy-food nexus to assess how the knowledge of SDG interlinkages has been operationalized to inform policymaking.

Performance Assessment of ELISA Using the Specific Antigen Tc323 for the Diagnosis of Chronic Chagas Disease.

In the chronic phase of Chagas disease (CCD), diagnosis relies on detecting specific IgG antibodies due to the low or absent presence of the parasite in human blood. However, the performance of current serological tests is highly variable, lacking a "" assay with 100% sensitivity and specificity, which challenges the exploration of new biomarkers. In the present study, we evaluated the diagnostic accuracy of an optimized ELISA using the predicted immunogenic domains (called TcD3 and TcD6) of Tc323, a protein highly conserved among strains but absent in other clinically significant parasites such as .