Mechanisms of PGF2 alpha action in functional luteolysis.

The luteolytic action of PGF2 alpha appears to be due to loss of gonadotropin support of corpus luteum function. This "chemical hypophysectomy" produced by PGF2 alpha in the rat has several components: 1. A rapid block of gonadotropin uptake in vivo by an unexplained mechanism.

Specific binding of prostaglandin F2 alpha to membranes of rat corpora lutea.

Prostaglandin F2 alpha (PGF2 alpha) binds specifically to a partially purified membrane preparation from rat corpora lutea. The high affinity, low capacity binding component asa a Kd = 4.7 nM and has a capacity of 0.

Action of gonadotropin-releasing hormone and its superactive analogues on the anterior pituitary: the mechanism of release and synthesis of gonadotropins.

The role of prostaglandins (PG), their active intermediates or the adenylcyclase-cyclic AMP system for gonadotropin release and/or synthesis was evaluated by administering gonadotropin-releasing hormone (Gn-RH) and its superactive analog to normal and aspirin-treated rats. Serum LH levels, anterior pituitary malondialdehyde (MDA) content and cyclic AMP (cAMP) levels were followed. The pituitaries stimulated with Gn-RH or its superactive analog yielded more MDA and cAMP than the controls.