Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics.

Single-cell RNA-sequencing (scRNA-seq) is becoming a ubiquitous method in profiling the cellular transcriptomes of both malignant and non-malignant cells from the human brain. Here, we present a protocol to isolate viable tumor cells from human ex vivo glioblastoma cultures for single-cell transcriptomic analysis. We describe steps including surgical tissue collection, sectioning, culturing, primary tumor cells inoculation, growth tracking, fluorescence-based cell sorting, and population-enriched scRNA-seq.

Wing morphology of a damselfly exhibits local variation in response to forest fragmentation.

Environmental differences can lead to morphologically different subpopulations. The scale of the mosaic of morphologies should help shed light on the nature of the mechanisms at work. Previous work has shown that jewelwing damselflies have different wing sizes in different types of habitat.

Therapeutic relationships in speech-language pathology: A scoping review of empirical studies.

Purpose: This scoping review provides an overview of empirical studies investigating therapeutic relationships between speech-language pathologists (SLPs), clients, and caregivers across all ages and clinical areas, and identifies areas of future research.

Method: The Joanna Briggs Institute's (JBI) scoping review method was employed. Systematic searches were conducted across seven databases and four grey literature databases.

Therapeutic relationships in speech-language pathology: a scoping review protocol.

Objective: The purpose of this scoping review is to provide an overview of empirical research regarding the therapeutic relationship between speech-language pathologists and their clients of all ages and clinical areas.

Introduction: The influence of a positive relationship between professionals and clients on the process and outcome of interventions is well documented for many health professions. However, research in speech-language pathology predominantly focuses on the outcome of specific treatment methods and techniques.

A 17-Year-Old Boy With High-Functioning Autism, Gastrointestinal Illness, and Seizures.

A healthy 17-year-old boy with a high-functioning pervasive developmental disorder presented to the emergency department after having a 4-minute episode of seizure-like activity in the setting of presumed viral gastroenteritis. Within an hour of emergency department arrival, he developed a forehead-sparing facial droop, right-sided ptosis, and expressive aphasia, prompting stroke team assessment and urgent neuroimaging. Laboratory results later revealed a serum sodium of 119 mmol/L.