Downregulation of POFUT1 Impairs Secondary Myogenic Fusion Through a Reduced NFATc2/IL-4 Signaling Pathway.

Past work has shown that the protein -fucosyltransferase 1 (POFUT1) is involved in mammal myogenic differentiation program. knockdown (Po -) in murine C2C12 cells leads to numerous elongated and thin myotubes, suggesting significant defects in secondary fusion. Among the few pathways involved in this process, NFATc2/IL-4 is described as the major one.

as a Promising Novel Biomarker of Colorectal Cancer.

Background: While protein -fucosyltransferase 1 () overexpression has been recently proposed as a potential biomarker for different cancer types, no study was carried out on POFUT1 implication in colorectal cancer (CRC).

Methods: Data from 626 tumors and 51 non-tumor adjacent tissues available in FireBrowse had been used in this study. Statistical analyses on expression and gene copy number, receptors (main targets of POFUT1 enzymatic activity) expression and association of and expressions with clinical parameters were investigated.

PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents-Synthesis, physicochemical characterization and in vitro evaluation.

We describe the synthesis of cellulose nanocrystals (CNCs) complexed to siRNA. PEI (600Da) was first covalently attached to CNCs by reductive amination reaction, then CNCs-PEI were loaded with siRNA, resulting in the formation of CNCs-PEI-siRNA particles, strongly stabilized by ionic interactions. Efficient cellular uptake of these particles was monitored by ethidium bromide staining.