Publications by authors named "J L Caplin"
Article Synopsis
- Immune checkpoint inhibitors, a type of cancer treatment, can cause serious side effects, including immune-related myocarditis (irMyocarditis), which can be fatal.
- Researchers studied immune responses in the heart, tumor, and blood of 28 patients with irMyocarditis using advanced techniques like single-cell RNA sequencing and T-cell receptor (TCR) sequencing.
- Their findings showed an increase in certain immune cells in the heart tissue of irMyocarditis patients and identified specific TCR clones associated with severe cases, shedding light on the disease's biology and potential biomarkers.
Article Synopsis
- Immune checkpoint inhibitors (ICIs) can lead to serious immune-related adverse events (irAEs), with ICI-related myocarditis (irMyocarditis) being particularly dangerous and the most lethal among these events.
- Researchers explored immune responses in the heart, tumors, and blood of 28 patients with irMyocarditis compared to 23 controls, using advanced techniques like single-cell RNA sequencing and proteomics.
- Key findings revealed a unique presence of specific immune cells in irMyocarditis heart tissue, distinct T cell responses in heart vs. tumor, and identified novel biomarkers related to fatality that could inform future therapies.
Diabetes is associated with an altered global inflammatory state with impaired wound healing. Mesenchymal stem/stromal cells (MSC) are being explored for treatment of diabetic cutaneous wounds due to their regenerative properties. These cells are commonly delivered by injection, but the need to prolong the retention of MSC at sites of injury has spurred the development of biomaterial-based MSC delivery vehicles.
View Article and Find Full Text PDFWe describe a facile strategy to identify sites for the incorporation of noncanonical amino acids into lysostaphin-an enzyme that degrades the cell wall of Staphylococcus aureus-while retaining stapholytic activity. We used this strategy to generate active variants of lysostaphin incorporating para-azidophenylalanine. The incorporation of this "reactive handle" enabled the orthogonal site-specific modification of the enzyme variants with polyethylene glycol (PEG) using copper-free click cycloaddition.
View Article and Find Full Text PDFObjective: To assess longitudinal variation in patterns of retromolar space growth, with regard to sex and cervical vertebrae maturation.
Design: We utilized serial lateral cephalograms from three craniofacial growth studies (Denver, Iowa, Oregon), measuring retromolar space and cervical vertebrae maturation in 99 subjects (56% male) from 8 to 18 years of age for each subject. Repeated measures ANOVA and a linear mixed effects model were used to assess retromolar space growth through time.