Consecutive Marcus Electron and Proton Transfer in Heme Peroxidase Compound II-Catalysed Oxidation Revealed by Arrhenius Plots.

Electron and proton transfer reactions in enzymes are enigmatic and have attracted a great deal of theoretical, experimental, and practical attention. The oxidoreductases provide model systems for testing theoretical predictions, applying experimental techniques to gain insight into catalytic mechanisms, and creating industrially important bio(electro)conversion processes. Most previous and ongoing research on enzymatic electron transfer has exploited a theoretically and practically sound but limited approach that uses a series of structurally similar ("homologous") substrates, measures reaction rate constants and Gibbs free energies of reactions, and analyses trends predicted by electron transfer theory.

Highly sensitive amperometric biosensor based on alcohol dehydrogenase for determination of glycerol in human urine.

In this paper we report the development of a highly sensitive amperometric glycerol biosensor based on alcohol dehydrogenase from Pseudomonas putida immobilized on graphite electrode modified with carbon nanotubes and a redox mediator tetrathiafulvalene. The designed biosensor demonstrates very high sensitivity towards glycerol (29.2 ± 0.

Bioanode with alcohol dehydrogenase undergoing a direct electron transfer on functionalized gold nanoparticles for an application in biofuel cells for glycerol conversion.

In this paper we designed and investigated bioanode with alcohol dehydrogenase (ADH) catalysing oxidation of glycerol and glyceraldehyde. The most effective bioanode was fabricated when ADH was immobilized on gold nanoparticles (AuNPs) modified with 4-aminothiophenol. This electrode catalysed the oxidation of both glycerol and glyceraldehyde thus demonstrating a consecutive two-step process.

Laccase-catalyzed bisphenol A oxidation in the presence of 10-propyl sulfonic acid phenoxazine.

The kinetics of the Coriolopsis byrsina laccase-catalyzed bisphenol A (BisA) oxidation was investigated in the absence and presence of electron-transfer mediator 3-phenoxazin-10-yl-propane-1-sulfonic acid (PPSA) at pH5.5 and 25°C. It was shown that oxidation rate of the hardly degrading compound BisA increased in the presence of the highly reactive substrate PPSA.

Evanescent-field-induced Raman scattering for bio-friendly fingerprinting at sub-cellular dimension.

Evanescent field induced chemical imaging concept has been realized in analytical platform based on the µ-tip-enhanced Raman scattering spectroscopy (µ-TERS). The technique aimed to minimize thermal decomposition of dried biological sample as the result of huge concentration of optical field near the tip by increasing the size of an aperture-less "excitation source". µ-TERS technique is similar to classical biosensor systems based on propagating surface plasmon resonance phenomenon but with sensitive elements a few micrometers in size that can be targeted to the area of interest.