Publications by authors named "J Kubovciak"
Article Synopsis
- - Our study investigates the origins of the vertebrate head using amphioxus, which shares traits with early chordates, by employing techniques like single-cell transcriptomics and signaling pathway analysis.
- - We identified different types of cells in amphioxus embryos that are similar to those involved in vertebrate head development, particularly focusing on prechordal plate-like and neural crest-like cells.
- - The research shows that key signaling pathways (Nodal, Hedgehog, and Wnt) are conserved in both vertebrates and amphioxus, suggesting that the mechanisms of head development can be traced back to a common ancestor of all chordates.
Dynamic changes in maternal‒zygotic transition (MZT) require complex regulation of zygote formation, maternal transcript decay, embryonic genome activation (EGA), and cell cycle progression. Although these changes are well described, some key regulatory factors are still elusive. Sirtuin-1 (SIRT1), an NAD-dependent histone deacetylase, is a versatile driver of MZT via its epigenetic and nonepigenetic substrates.
View Article and Find Full Text PDFAims: To investigate the therapeutic potential of visual stimulation (VS) and BDNF in murine experimental autoimmune uveoretinitis (EAU).
Main Methods: Mice were immunized by subcutaneous injection of interphotoreceptor retinoid-binding protein in Freund's complete adjuvant and intravenous injection of pertussis toxin, and were then exposed to high-contrast VS 12 h/day (days 1-14 post-immunization). EAU severity was assessed by examining clinical score, visual acuity, inflammatory markers, and immune cells in the retina.
The single-layer epithelium of the gastrointestinal tract is a dynamically renewing tissue that ensures nutrient absorption, secretory and barrier functions and is involved in immune responses. The basis for this homeostatic renewal is the Wnt signaling pathway. Blocking this pathway can lead to epithelial damage, while its abnormal activation can result in the development of intestinal tumors.
View Article and Find Full Text PDFStatins, the drugs used for the treatment of hypercholesterolemia, have come into the spotlight not only as chemoadjuvants, but also as potential stem cell modulators in the context of regenerative therapy. In our study, we compared the in vitro effects of all clinically used statins on the viability of human pancreatic cancer (MiaPaCa-2) cells, non-cancerous human embryonic kidney (HEK 293) cells and adipose-derived mesenchymal stem cells (ADMSC). Additionally, the effect of statins on viability of MiaPaCa-2 and ADMSC cells spheroids was tested.
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