Protein synthesis on membrane-bound and free ribosomes in growing yeast.

1. A method for the study of membrane-binding of ribosomes and the rate of synthesis of protein on free and bound ribosomes has been examined critically. 'Brij 58' has been found to be more suitable for detaching ribosomes from membranes than 'Triton X-100' or sodium deoxycholate since it does not alter the structure or biological activity of the ribosomes.