[A sensitive method for detection of sympathomimetic amines in pentafluorobenzamide form in biological samples using gas chromatography].

A toxicological method of sensitive and specific confirmation of methamphetamine and other primary and secondary amines in biological samples after extractive perfluorobenzoylation is described. The method is based on the principle of gas chromatography with parallel specific nitrogen detection and electron capture detection. The other alternative is the gas chromatography combined with mass spectrometry in chemical ionization mode.

Gas chromatography of simple phenols in biological fluids.

Acid hydrolysis of phenol conjugates in urine by concentrated H3PO4 followed by extraction of phenols with n-hexane and their acetylation before gas chromatography on columns packed with OV-1 or OV-17 is described. The sensitivity of the method is sufficient to monitor normal levels of phenol and p-cresol or phenol and o-cresol after exposure to benzene or toluene vapours. The detection limit is 1 mg/l.

Chlorinated phenols as metabolites of lindane. Evaluation of the degree of conjugation in rat urine.

After lindane was administered to rats in a single oral dose, the time profile of the degree of conjugation of the main phenolic metabolites was evaluated. In all urine samples 2,3-dichlorophenol, 2,4,5- and 2,4,6-trichlorophenol, and 2,3,5,6-tetrachlorophenol were constantly present. 2,3,5,6-Tetrachlorophenol and then 2,4,6-trichlorophenol were the prevalent metabolites in all cases.

Rapid determination of ethylene glycol at toxic levels in serum and urine.

The rapid gas chromatographic detection and determination of ethylene glycol in biological fluids is described. Phenylboronic acid in acetone was used for the esterification of glycol. The phenylboronates of ethylene glycol and 1,2-propylene glycol are not separated on a packed column of medium polarity (OV-17), but they can be separated on a non-polar column (OV-101).