Post-larvae movement network of marine shrimp during the 2013 outbreak of acute hepatopancreatic necrosis disease in Thailand.

This study aimed to assess the movement patterns of post-larvae (PL) of whiteleg shrimp and black tiger shrimp, which were linked to the epidemic of acute hepatopancreatic necrosis disease (AHPND) in Thailand in 2013, using social network analysis. Hatcheries or nurseries were assigned as origin nodes, whereas cultivated ponds were designated as destination nodes. From 376 farms, data of 1300 ponds were collected from network data using a questionnaire.

Risk factors associated with white spot syndrome virus outbreaks in marine shrimp farms in Rayong Province, Thailand.

The purpose of this study was to determine risk factors for white spot disease (WSD) in Rayong, Thailand. A study was conducted from October 2014 to March 2015 to identify potential farm-level risk factors using a validated questionnaire. We completed 1 questionnaire for each farm; a total of 38 questionnaires from the case farms and 127 questionnaires from the control farms were collected.

Impact of farm management on expression of early mortality syndrome/acute hepatopancreatic necrosis disease (EMS/AHPND) on penaeid shrimp farms in Thailand.

Asian shrimp farming industry has experienced massive production losses due to a disease caused by toxins of Vibrio bacteria, known as early mortality syndrome/acute hepatopancreatic necrosis disease (EMS/AHPND) for the last 5 years. The disease can cause up to 100% cumulative pond mortality within a week. The objective of this study was to identify factors associated with AHPND occurrence on shrimp farms.

Detection of yellow head virus in shrimp by loop-mediated isothermal amplification (LAMP).

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detecting the structural glycoprotein gene of yellow head virus (YHV). The RT-LAMP assay is a novel method of gene amplification that amplifies nucleic acid with high specificity, sensitivity and rapidity under isothermal conditions with a set of four specially designed primers that recognize six distinct sequences of the target. The whole procedure is very simple and rapid, and reaction time and temperatures were optimized for 60 min at 65 degrees C, respectively.

Progress in the development of shrimp cell cultures in Thailand.

Primary shrimp cell cultures were developed from lymphoid organ and ovaries of black tiger shrimp, Penaeus monodon, in double-strength Leibovitz's L-15 medium supplemented with 15% fetal bovine serum, 1% glucose, 5 g/L NaCl, 15% shrimp meat extract. The optimum conditions for primary culture in vitro were obtained in L-15 medium with an osmolality of approximately 730 +/- 10 mmol/kg, a temperature range of 25--28 degrees C and incubation in a normal atmosphere. However, basal medium supplemented with 0.