Partial purification and biological activity of the enterotoxic component of Pseudomonas aeruginosa.

From an Pseudomonas aeruginosa strain isolated from feces of a patient with clinical manifestation of diarrhoeal disease a filtrate from the culture in proteose peptone broth was prepared. After concentration, the culture filtrate was separated on Sephadex G-100 column in four fractions. The first high molecular fraction (F1) represented components of relative molecular mass exceeding 100,000, the second fraction those about 58,000, the third fraction those about 23,000 and the fourth one was composed from low molecular components of molecular mass lower than 10,000.