Programmable mRNA therapeutics for controlled epigenomic modulation of single and multiplexed gene expression in diverse diseases.

Pathogenic gene dysregulation can be attributed to chromatin state change that pre-transcriptionally regulates expression. Recent breakthroughs elucidating the rules governing this DNA control layer, an epigenetic code, unlock a modality in precision medicine to target gene dysregulation across myriad diseases. Here we present a modular platform to design programmable mRNA therapeutics, Epigenomic Controllers (EC), that control gene expression through directed epigenetic change.

Agent Orange and head and neck cancer: A systematic review and meta-analysis.

Objective: To assess the incidence of head and neck cancer in patients exposed to Agent Orange and related dioxins.

Methods: Studies were identified through CINAHL, PubMed, and Scopus. Primary studies were identified through April 2023.

Neurobehavioral Profiles in Opioid-Exposed and Unexposed Neonates.

Objective: To describe distinctive profiles of neurobehavior in opioid-exposed and unexposed neonates.

Study Design: The Outcomes of Babies with Opioid Exposure (OBOE) study is a multisite, prospective, observational study in neonates born at term with opioid exposure and unexposed controls. As part of the OBOE study protocol, certified examiners administered the NeoNatal Neurobehavioral Scales, 2 edition (NNNS-II) before 6 weeks postnatal age.

Autologous Bone Graft Harvest Technique Using Reamer-Irrigator-Aspirator for 2-Stage Revision Anterior Cruciate Ligament Reconstruction.

Autologous or allograft bone grafting is commonly used for bone restoration in the setting of revision anterior cruciate ligament (ACL) reconstruction owing to bone loss or malpositioned or osteolytic tunnels. For significant defects, staged procedures with initial bone grafting to the femoral and/or tibial tunnels is occasionally required. In this setting, allograft shows variable rates and quality of healing and can incur increased expense.

Supplementing Consent for a Prospective Longitudinal Cohort Study of Infants With Antenatal Opioid Exposure: Development and Assessment of a Digital Tool.

Background: The Outcomes of Babies With Opioid Exposure (OBOE) study is an observational cohort study examining the impact of antenatal opioid exposure on outcomes from birth to 2 years of age. COVID-19 social distancing measures presented challenges to research coordinators discussing the study at length with potential participants during the birth hospitalization, which impacted recruitment, particularly among caregivers of unexposed (control) infants. In response, the OBOE study developed a digital tool (consenter video) to supplement the informed consent process, make it more engaging, and foster greater identification with the research procedures among potential participants.