Publications by authors named "J K Muenzner"
Aneuploidy is a hallmark of human cancer, yet the molecular mechanisms to cope with aneuploidy-induced cellular stresses remain largely unknown. Here, we induce chromosome mis-segregation in non-transformed RPE1-hTERT cells and derive multiple stable clones with various degrees of aneuploidy. We perform a systematic genomic, transcriptomic and proteomic profiling of 6 isogenic clones, using whole-exome DNA, mRNA and miRNA sequencing, as well as proteomics.
View Article and Find Full Text PDFAneuploidy results in a stoichiometric imbalance of protein complexes that jeopardizes cellular fitness. Aneuploid cells thus need to compensate for the imbalanced DNA levels by regulating their RNA and protein levels, but the underlying molecular mechanisms remain unknown. In this study, we dissected multiple diploid versus aneuploid cell models.
View Article and Find Full Text PDFArticle Synopsis
- - Accessing the genetic diversity of species uncovers hidden traits and helps clarify gene functions, especially in natural isolates of Saccharomyces cerevisiae, where around 20% exhibit aneuploidy, which is contradicted by laboratory findings showing its fitness costs.
- - The research generates a proteomic resource for 796 euploid and aneuploid isolates, revealing that natural aneuploids have better protein dosage compensation compared to lab-generated ones, where many protein subunits show reduced expression.
- - Findings indicate that natural aneuploidy involves enhanced protein turnover and structural changes in the proteasome, suggesting that studying natural genetic diversity can provide valuable insights into the biological mechanisms behind aneupl
Gene expression varies between individuals and corresponds to a key step linking genotypes to phenotypes. However, our knowledge regarding the species-wide genetic control of protein abundance, including its dependency on transcript levels, is very limited. Here, we have determined quantitative proteomes of a large population of 942 diverse natural yeast isolates.
View Article and Find Full Text PDFUnlabelled: Gene expression varies between individuals and corresponds to a key step linking genotypes to phenotypes. However, our knowledge regarding the species-wide genetic control of protein abundance, including its dependency on transcript levels, is very limited. Here, we have determined quantitative proteomes of a large population of 942 diverse natural Saccharomyces cerevisiae yeast isolates.
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