The ongoing and contentious coverage of abortion in a progressive context: a long-term cross-outlet assessment of Dutch abortion news (2000-2022).

Utilising automated content analysis with transformer-based topic modelling (BERTopic), this study examines cross-outlet variation in volume and topics over two decades (2000-2022) of abortion coverage in seven major daily newspapers in the Netherlands. Our findings reveal an ongoing coverage of abortion. Volume of coverage was notably highest in religious newspapers, indicating that these newspapers constitute a prominent voice in Dutch abortion debate.

Quantifying uncertainty in method of moments estimates of the heterogeneity variance in random effects meta-analysis.

The between-study variance or heterogeneity variance is an important parameter in random effects meta-analysis. This paper uses an M-estimation framework to introduce and discuss variance estimators for quantifying the uncertainty in estimates of the heterogeneity variance using the noniterative generalized method of moments estimator and some related method of moments estimators. An example is used to further illustrate the variance estimators, and simulation results are presented for assessing the empirical properties of the proposed variance estimators.

Evaluating the Effects of Disinfectants on Bacterial Biofilms Using a Microfluidics Flow Cell and Time-Lapse Fluorescence Microscopy.

A commercially available microfluidics flow cell was utilized together with widefield fluorescence microscopy to evaluate the effects of disinfectants on bacterial strains. The flow cell's inner surface supports the formation of biofilms of numerous bacterial species. The modular setup of the flow cell accessories allows connection to syringes, pumps and collection vials, facilitating aseptic experiments in a controlled fluidics environment which can be documented with precisely timed microscopy imaging.

Author Correction: Guidance for quantitative confocal microscopy.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Tutorial: guidance for quantitative confocal microscopy.

When used appropriately, a confocal fluorescence microscope is an excellent tool for making quantitative measurements in cells and tissues. The confocal microscope's ability to block out-of-focus light and thereby perform optical sectioning through a specimen allows the researcher to quantify fluorescence with very high spatial precision. However, generating meaningful data using confocal microscopy requires careful planning and a thorough understanding of the technique.