Protein and mRNA expression of CD56/N-CAM on follicular epithelial cells of the human thyroid.

In order to confirm CD56/N-CAM antigen prevalence in the human thyroid and to compare its expression on thyrocytes and NK cells, an expression of CD56/N-CAM antigen was searched for on isolated thyroid follicular cells and NK cells by flow cytometry. In addition, mRNA for CD56 was searched for in RNA isolated from human thyroid samples and few other organs using dot blot hybridization assay to prove the existence of mechanisms for active synthesis of the protein in question. The isolated cells from the follicular epithelium of 22 various pathological thyroid tissue specimens were examined for the expression of CD56/N-CAM in terms of the percentage of positive cells and the mean fluorescence intensity (MFI).

[Ticks Ixodes ricinus (Linnaeus, 1758) from selected municipal forests of the city Poznań and their infection with the spirochetes Borrelia burgdorferi senso lato].

The results of research (1997-1998) on the occurrence of the spirochetes Borrelia burgdorferi s.l. in ticks Ixodes ricinus (L.

[Incidence of Borrelia burgdorferi sensu lato spirochetes in Ixodes ricinus ticks from a museum collection evaluated by PCR].

Ixodes ricinus ticks (total n = 51:14 larvae, 16 nymphs, 17 females and 11 males) collected at 21 different sites during 1948-1987 were examined individually for the presence of Borrelia burgdorferi sensu lato spirochaetes. Detection of spirochaetes was carried out by polymerase chain reaction (PCR) using primers complementary to chromosomal sequence. Borreliae were found in 3 (3.

[The evaluation of Borrelia burgdorferi sensu lato spirochaetes distribution in Ixodes ricinus ticks collected in selected regions of Wielkopolska+ regions by polymerase chain reaction (PCR) method].

Ixodes ricinus ticks (total n = 298: 24 nymphs, 156 females and 112 males) collected from vegetation at 7 different sites were examined individually for the presence of Borrelia burgdorferi sensu lato spirochaetes. Detection of spirochaetes was carried out by polymerase chain reaction (PCR) using primers complementary to chromosomal sequence. Borreliae were evident in 73 (24.