Publications by authors named "J Jarczak"
Populations of very small embryonic-like stem cells (VSELs) (CD34+lin-CD45- and CD133+lin-CD45-), circulating in the peripheral blood of adults in small numbers, have been identified in several human tissues and together with the populations of hematopoietic stem cells (HSCs) (CD34+lin-CD45+) and CD133+lin-CD45+constitute a pool of cells with self-renewal and pluripotent stem cell characteristics. Using advanced cell staining and sorting strategies, we isolated populations of VSELs and HSCs for bulk RNA-Seq analysis to compare the transcriptomic profiles of both cell populations. Libraries were prepared from an extremely small number of cells; however, their good quality was preserved, and they met the criteria for sequencing.
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- Researchers identified a specific population of Very Small Embryonic-Like Stem Cells (VSELs) in human postnatal tissues such as bone marrow, peripheral blood, and umbilical cord blood that can differentiate into various tissue stem cells.
- Molecular analysis showed that these VSELs share similar characteristics with embryonic stem cells, including certain gene expressions and patterns of genetic imprinting.
- Using single-cell RNA sequencing, the study found that these human VSELs also exhibit subpopulations related to germline development and are influenced by factors that control differentiation and immune response.
Purinergic signaling is an ancient primordial signaling system regulating tissue development and specification of various types of stem cells. Thus, functional purinergic receptors are present in several types of cells in the body, including multiple populations of stem cells. However, one stem cell type that has not been evaluated for expression of purinergic receptors is very small embryonic stem cells (VSELs) isolated from postnatal tissues.
View Article and Find Full Text PDFBackground: Molar incisor hypomineralization (MIH) is a qualitative, demarcated enamel defect of hypomineralization affecting one to four first permanent molars, often with incisor involvement. Its etiology is complex. However, evidence suggests the influence of genetic factors, potentially including the single nucleotide polymorphisms (SNPs) rs2889956, rs4811117 and rs13058467, which were previously linked to MIH in a genome-wide association study of German children.
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- - Mastocytosis is a disease linked to mast cells that can vary in severity, with the presence of the KIT p. D816V mutation playing a key role in its development, alongside potentially significant epigenetic factors that influence gene expression.
- - The study aimed to investigate differences in DNA methylation profiles between patients with indolent systemic mastocytosis (ISM) and healthy individuals, analyzing blood samples to identify regions of differing methylation (DMRs) that could indicate disease progression and management strategies.
- - Through analysis using a MethylationEPIC BeadChip kit and statistical methods like Limma, researchers assessed co-methylated regions to establish potential epigenetic predictors that could aid in understanding and treating mast