Sterically stabilized liposomes in cancer therapy and gene delivery.

The introduction of colloidally stable liposomes with low drug leakage rates resulted in a renaissance in liposome applications in cancer therapy. Furthermore, a platform of sterically stabilized liposomes also allows the construction of new generations of drug delivery vehicles. These include targeted liposomes and targeted nucleic acid delivery vehicles, based either on cationic sterically stabilized liposomes or pre-condensed DNA encapsulated in neutral or negatively charged liposomes.

Mitochondrial function in cell wall glycoprotein synthesis in Saccharomyces cerevisiae NCYC 625 (Wild type) and [rho(0)] mutants.

We studied phosphopeptidomannans (PPMs) of two Saccharomyces cerevisiae NCYC 625 strains (S. diastaticus): a wild type strain grown aerobically, anaerobically, and in the presence of antimycin and a [rho(0)] mutant grown aerobically and anaerobically. The aerobic wild-type cultures were highly flocculent, but all others were weakly flocculent.

Evaluation of the performance of controlled release dosage forms of ticlopidine using in vitro intestinal permeability and computer simulations.

Prototype controlled release formulations of ticlopidine hydrochloride were developed, but when administered to humans, these formulations significantly reduced the bioavailability of intact drug in plasma. In order to examine the intestinal permeability characteristics and gastrointestinal metabolism of 14C-ticlopidine, we employed an in vitro diffusion cell system to directly measure the permeation of ticlopidine across various segments of monkey and rabbit intestine. High pressure liquid chromatography was used to determine the amount of intact ticlopidine on both the mucosal and serosal sides of the intestinal tissue.