A Simplified Guide RNA Synthesis Protocol for SNAP- and Halo-Tag-Based RNA Editing Tools.

SNAP-tag and Halo-tag have been employed to achieve targeted RNA editing by directing the deaminase domain of human ADAR to specific sites in the transcriptome. This targeting is facilitated by short guide RNAs (gRNAs) complementary to the target transcript, which are chemically modified with benzylguanine or chloroalkane moieties to enable covalent binding to the respective self-labeling enzymes. However, broad application of this approach has been limited by challenges such as low scalability, the requirement for specialized chemical expertise and equipment, and labor-intensive protocols.

Supratherapeutic Inhaled Corticosteroid Use in Patients Initiating on Biologic Therapies for Severe Asthma: A Nationwide Cohort Study.

Background: In severe asthma, intensive ("supratherapeutic") doses of inhaled corticosteroids (ICS) are often used. The prevalence of supratherapeutic ICS use and its impact on corticosteroid-related comorbidities is poorly understood. We aimed to describe the prevalence of supratherapeutic ICS use in severe asthma, its relation to corticosteroid-related comorbidities, and changes in prescribed and redeemed ICS dose after 12 months of biologic therapy.

The impact of biologic therapy on work capacity and workforce attachment in patients with severe asthma.

Introduction: Severe asthma impacts work capacity, but little is known about the effects of biologic therapy on patients' ability to work. We aimed to assess the impact of biologic therapy for severe asthma on work capacity and workforce attachment.

Methods: This cohort study used the Danish Severe Asthma Register, comprising all Danish patients with severe asthma initiating biologic therapy.

Multimodal deep learning fusion of ultrafast-DCE MRI and clinical information for breast lesion classification.

Background: Breast cancer is the most common cancer worldwide, and magnetic resonance imaging (MRI) constitutes a very sensitive technique for invasive cancer detection. When reviewing breast MRI examination, clinical radiologists rely on multimodal information, composed of imaging data but also information not present in the images such as clinical information. Most machine learning (ML) approaches are not well suited for multimodal data.

The effect of lingual orthodontic appliances in the dimensional reduction of labial gingival recession and root prominence caused by the wire syndrome in the anterior mandible: a multicenter study.

Background: The wire syndrome (WS) or X-effect/twist-effect describes undesired long-term tooth movements following fixed retainer placement. Since it includes root torque changes that might cause gingival recession, those situations often require periodontal, orthodontic and conservative treatment. Aim of this study was to assess the effectiveness of fixed orthodontic treatment with completely customized lingual appliances (CCLA) and continuous archwires for a clinically significant reduction in the dimensions of labial gingival recessions in the anterior mandible, caused by the wire syndrome (WS), in a group of consecutive patients treated with the same approach.