Transposon sequencing reveals the essential gene set and genes enabling gut symbiosis in the insect symbiont .

is a bacterium belonging to the genus , which is able to colonize multiple environments like soils and the gut of the bean bug . We constructed a saturated mariner transposon library and revealed by transposon-sequencing that 498 protein-coding genes constitute the essential genome of for growth in free-living conditions. By comparing essential gene sets of and seven related strains, only 120 common genes were identified, indicating that a large part of the essential genome is strain-specific.

Dissimilar gene repertoires of involved in the colonization of lesions and roots of .

and species are necrotrophic pathogens that macerate stems (blackleg disease) and tubers (soft rot disease) of . They proliferate by exploiting plant cell remains. They also colonize roots, even if no symptoms are observed.

Dual-Uptake Mode of the Antibiotic Phazolicin Prevents Resistance Acquisition by Gram-Negative Bacteria.

Phazolicin (PHZ) is a peptide antibiotic exhibiting narrow-spectrum activity against rhizobia closely related to its producer, sp. strain Pop5. Here, we show that the frequency of spontaneous PHZ-resistant mutants in Sinorhizobium meliloti is below the detection limit.

Differential Genetic Strategies of Burkholderia vietnamiensis and Paraburkholderia kururiensis for Root Colonization of Oryza sativa subsp. and O. sativa subsp. , as Revealed by Transposon Mutagenesis Sequencing.

Burkholderia vietnamiensis LMG10929 and Paraburkholderia kururiensis M130 are bacterial rice growth-promoting models. Besides this common ecological niche, species of the genus are also found as opportunistic human pathogens, while species are mostly environmental and plant associated. In this study, we compared the genetic strategies used by B.