Complete genome sequence of bacteriophage Godfather isolated from .

Microbacteriophage Godfather was collected from a soil sample in Stephenville, Texas. The 17,452-bp double-stranded genome contains 24 protein-coding genes. The genome shares >99% nucleotide sequence identity with cluster EE microbacteriophages Scamander, Danno, Kojax4, and Burgy.

Exploring the Feasibility and Acceptability of a Brief Online Dialogic Book-Sharing Training for Teaching Support Staff.

Background/objectives: In the UK, significant and rising numbers of children arrive in schools with marked deficits in key skills such as oral language. This rise has been further negatively impacted by the COVID-19 pandemic. Given this, the foundation phase of primary school education is a necessary environment for targeting language deficits.

PFOA, PFOS and PFHxS toxicokinetic considerations for the development of an in vivo approach for assessing PFAS relative bioavailability in soil.

A Sprague-Dawley rat model was utilized to elucidate perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) toxicokinetics with a goal of developing an in vivo approach for quantifying PFAS relative bioavailability in impacted soil. Following single dose administration (gavage) of ∼ 0.2-2000 µg kg BW of PFOA, PFOS or PFHxS, differences in PFAS blood, organ and excreta concentrations were observed over 120 h although linear dose responses were determined for area under the blood plasma time curves (AUC; PFOA, PFHxS), liver accumulation (LA: PFOS) and urinary excretion (UE; PFOA, PFHxS).

Use of osteogenic bone matrix in patients with traumatic long bone defects: An open label, single center study.

Background: Osteogenic Bone Matrix (Altis™ OBM) is a tissue-engineered, porcine-derived demineralized bone matrix prepared using a humanization processing technology that confers biocompatibility and improved osteoinductivity. The objective of this study was to determine the safety and efficacy of OBM in patients with traumatic long bone defects in an open-label, non-randomized single-center study.

Methods: Diagnosis and main criteria for inclusion were open long bone fractures graded as Gustilo-Anderson Grade II, IIIA or IIIB.

Quantitative Spatial Analysis of Chromatin Biomolecular Condensates using Cryo-Electron Tomography.

Phase separation is an important mechanism to generate certain biomolecular condensates and organize the cell interior. Condensate formation and function remain incompletely understood due to difficulties in visualizing the condensate interior at high resolution. Here we analyzed the structure of biochemically reconstituted chromatin condensates through cryo-electron tomography.