Enhanced Specificity in Colorimetric LAMP Assay for Detection Using a Combination of Two Additives.

The genus comprises fungal species closely related to , with and being medically important. These species can cause infections in both immunocompetent and immunocompromised individuals. The current detection methods are limited, prompting the need for rapid and specific diagnostic tools.

Genome-wide conditional degron libraries for functional genomics.

Functional genomics with libraries of knockout alleles is limited to non-essential genes and convoluted by the potential accumulation of suppressor mutations in knockout backgrounds, which can lead to erroneous functional annotations. To address these limitations, we constructed genome-wide libraries of conditional alleles based on the auxin-inducible degron (AID) system for inducible degradation of AID-tagged proteins in the budding yeast Saccharomyces cerevisiae. First, we determined that N-terminal tagging is at least twice as likely to inadvertently impair protein function across the proteome.

An eMERGEing definition of patient engagement in genetic counseling.

The concept of patient engagement has been widely studied for decades in the fields of medicine, nursing, psychology, social science, public health, and policy, and increased levels of patient engagement have been shown to improve health outcomes and strengthen reported experiences of care. Despite this, little research has been done to evaluate what patient engagement looks like within the context of a genetic counseling session. Additionally, there is limited literature from researchers based in the United States that aims to better understand patient engagement in non-English-speaking populations.

Direct radiative effects of black carbon and brown carbon from Southeast Asia biomass burning with the WRF-CMAQ two-way coupled model.

Black carbon (BC) and brown carbon (BrC) are significant light-absorbing components of particulate matter that impact weather and climate. Biomass burning (BB) and biofuel (BF) emissions in Southeast Asia are key global sources of BC and BrC. This study utilizes the Weather Research and Forecasting-Community Multiscale Air Quality (WRF-CMAQ) model, integrating a BrC module for the first time, alongside the Global Fire Emissions Database Version 4, to assess the direct radiative effect (DRE) of BC and BrC in March 2015 over Southeast Asia.

Prospect of emerging treatments for HBV functional cure.

Functional cure, defined as sustained hepatitis B surface antigen (HBsAg) seroclearance with unquantifiable hepatitis B virus (HBV) DNA at 24 weeks off treatment, is a favorable treatment endpoint in chronic hepatitis B (CHB). Nonetheless, functional cure is rarely attained with the current treatment modalities of nucleos(t)ide analogues (NUCs) and pegylated interferon alpha (Peg-IFNα). Multiple novel virus-targeting agents and immunomodulators are under development for HBV with functional cure as the treatment goal.