Structure of a putative terminal amidation domain in natural product biosynthesis.

Bacteria are rich sources of pharmaceutically valuable natural products, many crafted by modular polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS). PKS and NRPS systems typically contain a thioesterase (TE) to offload a linear or cyclized product from a carrier protein, but alternative chemistry is needed for products with a terminal amide. Several pathways with amidated products also possess an uncharacterized 400-amino acid terminal domain.

Implementing Australia's new guidelines for appropriate psychotropic medication use in residential aged care: Applying the Consolidated Framework For Implementation Research.

Objectives: Accumulating evidence about psychotropic medication-related adverse events has had minimal apparent impact on overall rates of psychotropic medication use across Australian residential aged care facilities (RACFs). Australia's new Clinical Practice Guidelines for the Appropriate Use of Psychotropic Medications in People Living with Dementia and in Residential Aged Care were released in April 2023. This study aimed to identify contextual factors to inform strategies to implement the new Guidelines in Australian RACFs.

A Dinuclear Nickel Complex for Electrocatalytic Nitrite Reduction.

While many mononuclear complexes for electrocatalytic NO reduction have been reported, there are few examples of dinuclear electrocatalysts. Here, we report on the electroreduction of NO by the dinuclear complex [Ni(tpmc)(NO)] (tpmc = 1,4,8,11-tetrakis-(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane) and a mononuclear analogue [Ni(cyclam)] (cyclam = 1,4,8,11-tetraazacyclotetradecane). Notably, the presence of the second metal ion in [Ni(tpmc)(NO)] shifts the onset of catalysis anodically by ca.

Predicting White Matter Hyperintensity: Leveraging Portable Magnetic Resonance Imaging for Accessible Brain Health Screening.

Background And Purpose: Portable MRI (pMRI) has emerged as a cost-effective and accessible tool for the identification of white matter hyperintensities (WMH), an independent risk factor for stroke and dementia. Our objective was to confirm that pMRI can produce accurate WMH measurements and to develop and validate a risk model to predict WMH on pMRI for the purpose of identifying patients who may benefit from pMRI screening.

Materials And Methods: The development (N=143) and validation (N=127) cohorts included patients without acute neurologic pathology who received a pMRI at a tertiary care hospital between May 2020 and July 2024.

Plasma metabolite profiles of meat intake and their association with cardiovascular disease risk: A population-based study in Swedish cohorts.

Background: Higher meat intake has been associated with adverse health outcomes, including cardiovascular disease (CVD). This study investigated plasma metabolites associated with meat intake and their relation with cardiometabolic biomarkers, subclinical CVD markers, and incident CVD.

Methods: Associations between self-reported meat intake and 1272 plasma metabolites were investigated in the SCAPIS cohort (n = 8819; ages 50-64).