A human lymphokine released by mononuclear blood cells upon contact with CEA.

As revealed by the macrophage electrophoretic mobility (MEM) technique mononuclear blood cells from certain cancer patients respond to carcinoembryonic antigen (CEA). This phenomenon appeared to be due to a specific lymphokine release. In this study, the lymphokine activity of supernatant pools was stepwise enriched by gel filtration on Sephadex.

Charge-reducing lymphokine activity in Concanavalin A-stimulated lymphocyte supernatants: preliminary biological and biochemical characterization.

Supernatants of Concanavalin A stimulated (active) and unstimulated (control) guinea pig lymphocytes were fractionated by gel filtration on Sephadex G-100 into four arbitrary fractions (F1-F4). Fraction F3 from active supernatant (m.w.

Human lymphocyte response to CEA: titration of different CEA samples and neutralization experiments with monoclonal anti-CEA antibodies.

By means of the macrophage electrophoretic mobility technique a striking digestive system cancer-associated human lymphocyte response to CEA has been found during a large-scale study including tests in 499 individuals. The question to be answered by this study was whether this response is really CEA-specific. Titration experiments with 3 different CEA preparations in lymphocytes from 5 colorectal cancer patients showed that the threshold dose of CEA necessary to induce lymphocyte responses amounts to 50-100 ng CEA per ml and 10(6) lymphocytes, regardless of the CEA origin and its state of purity.

[Discrimination of nonspecific effects in the macrophage-electrophoretic-mobility test using dimethyl sulfoxide].

Upon incubation with human encephalitogenic protein (HEP) blood lymphocytes from patients with malignant tumours release mediators (lymphokines) leading to a decreased electrophoretic mobility of guinea pig peritoneal macrophages. The lymphocyte supernatants used for incubating the macrophages contain HEP, nonspecific lymphocyte-derived proteins, and in case of sensitized lymphocytes also specific mediators. Whereas HEP or nonspecific lymphocyte products do not themselves exert any effect on macrophages, they produce a nonspecific mobility reduction when acting simultaneously.

Reactivity of human lymphocytes to teratoma preparations as revealed by the macrophage electrophoretic mobility (MEM) technique.

By means of the MEM technique, the lymphokine release by human lymphocytes has been measured after exposure to 3M KCl extracts from malignant teratoma tissue. Clear-cut responses were found in 149 out of 171 cancer patients (87%) regardless of the primary cancer site. In contrast, only 15 out of 93 (16%) individuals from a control group without detectable malignant disease gave such a response.