Use of a FLAER-based WBC assay in the primary screening of PNH clones.

Diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) with flow cytometry traditionally involves the analysis of CD55 and CD59 on RBCs and neutrophils. However, the ability to accurately detect PNH RBCs is compromised by prior hemolysis and/or transfused RBCs. Patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS) can also produce PNH clones.

Evaluation of antithyroglobulin antibodies after routine vaccination in pet and research dogs.

Objective: To determine whether routine vaccination induces antibodies against bovine thyroglobulin and autoantibodies against canine thyroglobulin in dogs.

Design: Prospective study.

Animals: 20 healthy research Beagles and 16 healthy pet dogs.

Effects of vomitoxin ingestion on murine models for systemic lupus erythematosus.

Dietary exposure to the trichothecene vomitoxin (VT) results in reduced body weight gain, elevated serum IgA, terminal differentiation of Peyer's patch B cells to IgA secreting plasma cells haematuria, and increased kidney mesangial IgA accumulation in B6C3F1 mice and other inbred strains. These effects closely mimic a human autoimmune-like kidney disease known as IgA nephropathy. Using NZBW/F1, MRL/lpr, and BXSB mouse strains as models of systemic lupus erythematosus, we assessed whether consumption of diet containing 5 ppm or 10 ppm VT will similarly affect mice genetically prone to autoimmunity.

Effects of intermittent vomitoxin exposure on body weight, immunoglobulin levels and haematuria in the B6C3F1 mouse.

Continuous dietary exposure of female B6C3F1 mice to the trichothecene vomitoxin (VT) results in reduced body weight gain, elevated production of serum immunoglobulin A (IgA), kidney mesangial IgA deposition and glomerulonephritis. To assess whether intermittent consumption of dietary VT, as might occur during natural animal and human exposures, has similar effects to those for continuous consumption, a comparison was made between two schedules of dietary exposure. Female B6C3F1 mice were fed for 13 weeks with either a semipurified AIN-76A diet containing 20 ppm VT continuously or with 20 ppm VT intermittently (every other week).