Proposal of a Subclassification of pN3 in Squamous Cell Carcinoma of the Penis.

Objective: To evaluate the differential prognosis of PLNM as compared to ENE and postulate a sub-classification of the pN3 stage of carcinoma penis.

Methods: A retrospective analysis of prospectively maintained data of all the patients of penile squamous cell carcinoma operated at our institute between Jan 2016 and May 2023 was conducted. LASSO Cox regression was used to select variables affecting OS.

Distinct mobility patterns of BRCA2 molecules at DNA damage sites.

BRCA2 is an essential tumor suppressor protein involved in promoting faithful repair of DNA lesions. The activity of BRCA2 needs to be tuned precisely to be active when and where it is needed. Here, we quantified the spatio-temporal dynamics of BRCA2 in living cells using aberration-corrected multifocal microscopy (acMFM).

Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores.

Single-molecule localization microscopy (SMLM) uses activatable or switchable fluorophores to create non-diffraction limited maps of molecular location in biological samples. Despite the utility of this imaging technique, the portfolio of appropriate labels for SMLM remains limited. Here, we describe a general strategy for the construction of "glitter bomb" labels by simply combining rhodamine and coumarin dyes though an amide bond.