Publications by authors named "J Horiuchi"
Development of a novel and broadly applicable sandwich ELISA assay based on rabbit single-chain variable fragments and a modified Ig-binding domain of protein L fused to a polystyrene-binding peptide.
J Immunol Methods
November 2024
Most of currently available sandwich-type enzyme-linked immunosorbent assays (ELISA) require the use of full-length animal-derived antibodies which poses welfare criticisms and are often expensive to produce. There is therefore a strong demand for the development of more affordable and animal-free methods to produce antibodies for sandwich ELISA assay. To address these issues, we propose here the development of a new technology based on two complementary rabbit single-chain variable fragments (scFvs) and an Ig-binding domain of protein L (PpL1) fused to a polystyrene-binding peptide (PS-tag) that can be recombinantly produced in bacteria.
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- Haploinsufficiency of the ZBTB18/RP58 transcriptional repressor is linked to intellectual disability, but its underlying mechanisms and treatments are not well understood.
- Research using heterozygous-knockout mice showed early memory impairments and increased DNA/mitochondrial damage compared to wild-type mice, indicating that ZBTB18/RP58 is important for DNA repair.
- Treatment with minocycline, which has neuroprotective effects, was found to reduce inflammation and cognitive decline in these mice, suggesting its potential as a therapy for cognitive dysfunction related to ZBTB18/RP58 deficiency.
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- The sympathetic nervous system reacts to psychological stress by raising blood pressure, a condition that can persist even after the stress is gone, potentially contributing to hypertension.
- Researchers investigated how astrocytes, a type of brain cell, influence this ongoing rise in blood pressure using arundic acid, an inhibitor of astrocytes.
- The study found that arundic acid reduced the blood pressure increase caused by stress in rats, indicating that both neurons and astrocytes are important in understanding stress-related hypertension.
Structural adhesives are currently applied in the assembly of automobiles, aircraft, and buildings. In particular, epoxy adhesives are widely used due to their excellent mechanical strength and durability. However, cured epoxy resins are typically rigid and inflexible; thus, they have low peel and impact strength.
View Article and Find Full Text PDFIn this study, we have demonstrated a complementary-determining region (CDR) grafting technology for the generation of rabbit scFvs with different antigen recognition and physicochemical properties. The antigen-binding affinity of the CDR-grafted anti-CRP scFv, C1R/B1R (V1), which was generated by the CDR/framework region (CDR/FR) definition based on the traditional numbering rule, was insufficient when compared to that of the original clone, C1R, suggesting that the amino acid residues outside the original CDRs might significantly contribute to antigen recognition in rabbit scFvs. We redefined new CDRs and FRs to maintain antigen-binding affinities through the extension of multiple amino acid residues for CDRH1 and CDRH2, based on the amino acid sequence alignments of rabbit scFvs isolated from phage libraries.
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