A human in vitro model of bladder tumour cell invasion.

To study invasion of transitional cell cancer three-dimensional coculture assay consisting of living human tissues has been developed. Multicellular spheroids initiated from an invasive human bladder tumour cell line (Hu-1703He) were confronted with precultured fragments derived from normal human bladder mucosa. The fragments consisted of a surface epithelium and a central stroma.

Nonadheshive stationary organ culture of normal human urinary bladder mucosa.

Cold cup biopsies from normal human bladder mucosa were taken at cystoscopy. They were cut into fragments of 300-450 microns and grown in suspension in microwells base coated with agar. Within the first 24 hours of culture the fragments were totally covered by epithelium.

The prognostic value of p53 in superficially infiltrating transitional cell carcinoma.

Approximately 1/3 of T1 bladder cancers treated by endoscopic resection alone will progress. Prognostic factors are needed to help selecting appropriate treatment for these tumors. The purpose of the present investigation was to study the relation between p53 nuclear overexpression and disease progression.

Flow cytometric DNA and p53 analysis in superficially infiltrating bladder carcinoma.

Mutations in the p53 gene occur frequently in bladder cancers. Better prognostic factors are needed to help select appropriate treatment for patients with TCC stage T1. Paraffin-embedded tumors from 73 patients with TCC stage T1 were processed for two-parameter flow cytometry, measuring both p53 protein and DNA.

Relation between flow cytometric DNA distribution and pathology in human bladder cancer. A report on 69 cases.

High-resolution flow cytometric measurements of cellular DNA content have been performed in 69 patients with transitional cell carcinomas of the urinary bladder. By selective sampling of cells at cystoscopy, mapping of the whole bladder could easily be performed, including the tumor as well as different areas of the surrounding normal-appearing mucosa. The cell-cycle distribution showed an increasing fraction of cells with S- and G2-phase content parallel to the World Health Organization (WHO) grade of the tumors, ranging from 1.