Publications by authors named "J Higuchi"
Osteoarthritis (OA) shows various clinical manifestations depending on the status of its joint components. We aimed to identify the synovial cell subsets responsible for OA pathophysiology by comprehensive analyses of human synovium samples in single-cell resolution. Two distinct OA synovial tissue groups were classified by gene expression profiles in RNA-Seq: inflammatory and fibrotic.
View Article and Find Full Text PDFIntroduction: This study aimed to determine the association between the baseline magnetic resonance imaging (MRI) findings and clinical outcomes after articular injection of adipose-derived mesenchymal stem cells (ASCs) for knee osteoarthritis (KOA).
Methods: This retrospective study included 149 patients with varus-type KOA treated with a single intraarticular ASC injection. All patients underwent a MRI evaluation before treatment.
Sialyl glycans have several biological functions. We have previously reported on the preparation and bifidogenic activity of milk-derived sialylglycopeptide (MSGP) concentrate containing sialyl -glycans. The current study qualitatively and quantitatively analyzed the sialyl -glycans present in the MSGP concentrate.
View Article and Find Full Text PDFCritical-size bone defects necessitate bone void fillers that should be integrated well and be easily vascularized. One viable option is to use a biocompatible synthetic polymer and sonocoat it with zinc oxide (ZnO) nanoparticles (NPs). However, the ideal NP concentration and size must be assessed because a high dose of ZnO NPs may be toxic.
View Article and Find Full Text PDFArticle Synopsis
- Human milk is rich in carbohydrates, specifically human milk oligosaccharides (HMOs) and various types of glycans, but the specific profile of glycans had not been thoroughly studied until now.
- This study employed label-free chromatography-mass spectrometry (LC-MS) to analyze the variation in glycan composition and concentration in 200 samples of Japanese human milk from mothers 1-2 months postpartum.
- Findings showed that the presence of fucose linked to galactose affected the abundance of certain glycans and HMOs, with secretor mothers producing more of these compounds compared to nonsecretors, although the overall profile of glycans remained unchanged.