Determination of phencyclidine by radioimmunoassay.

A radioimmunoassay (RIA) was developed for measurement of phencyclidine (PCP) and its monohydroxy metabolites in urine. Anti-PCP serum was generated in rabbits and the labeled antigen was prepared by radio-iodination (125I) by the Hunter-Greenwood procedure. Adjustment of the antiserum and 125I-antigen concentrations resulted in a dynamic response from 0 to 200 ng/ml and as little as 2 ng/ml was detected.

Detection of barbiturates by latex agglutination inhibition.

A latex-agglutination inhibition test was developed and evaluated for the detection of barbiturates, primarily in urine, but it has applicability to serum. The 2-h test was performed with samples free of gross debris in a 37 degree C heat block and the reaction between the barbiturate antibody and the barbiturate-latex, at pH 7.3, was inhibited by urines containing secobarbital, amobarbital, pentobarbital, butabarbital, or phenobarbital.

Detection of drugs of abuse by radioimmunoassay: a summary of published data and some new information.

We review the status of radioimmunoassays for detection of abused drugs. Individual assays with use of 125I-labeled antigens are all performed in an identical manner and can be completed in 30 min to 1 h. Combined assays for simultaneous detection of two or more such drugs or assays in which a tritium-labeled antigen is used require 1-2 h for completion.

Iodination of morphine and dihydromorphine, as related to radioimmunoassay.

Direct iodination of morphine or dihydromorphine with iodine-125 results in products that bind to antibody raised by immunization of animals with a 3-O-carboxymethylmorphine/bovine serum albumin immunogen. Iodination of morphine or dihydromorphine with iodine-127 under identical conditions failed to yield these immunologically active products, suggesting that the results obtained with iodine-125 are in part due to a radiolytic reaction not observed with the iodine-127.