Uterine Transplant Optimization From a Preclinical Donor Model With Controlled Cardiocirculatory Arrest.

Background: Uterus transplantation from deceased donors offers a promising solution to the organ shortage, but optimal preservation methods are crucial for successful outcomes. Our primary objective is to conduct an initial assessment of the contribution of oxygenated hypothermic perfusion in uterine transplantation.

Methods: We performed a preclinical study on a porcine model of controlled donation after circulatory death (60 min warm ischemia).

Pancreas Preservation: Hypothermic Oxygenated Perfusion to Improve Graft Reperfusion.

Background: The clinical standard for pancreas preservation for transplantation is static cold storage (SCS). Oxygenation during preservation has been shown to be advantageous in clinical studies. This study evaluates the efficiency of different oxygenation modalities during hypothermic pancreas preservation.

Cortex kidney tissue partial oxygen pressure depends on percentage of active oxygenation during oxygenated hypothermic machine perfusion.

Background: Preclinical and clinical studies have demonstrated the advantages of oxygenated hypothermic machine perfusion (HMPO) during kidney preservation. However, the optimal oxygenation levels during HMPO remain undetermined. The aim of this study was to compare different levels of oxygenation (0%, 21%, 50%, and 100%) during 22 h of active oxygenated HMP (HMPO) using oxygen preloading by bubbling oxygenation in the preservation solution and continuous surface oxygenation during MP in a porcine DCD model.

MHC Class I Masking to Prevent AMR in a Porcine Kidney Transplantation Model in Alloimmunized Recipients.

Unlabelled: Presensitized patients awaiting a kidney transplant have a lower graft survival and a longer waiting time because of the limited number of potential donors and the higher risk of antibody-mediated rejection (AMR), particularly in the early posttransplant period, because of preformed donor-specific antibodies binding major histocompatibility complex (MHC) molecules expressed by the graft endothelium followed by the activation of the complement. Advances in kidney preservation techniques allow the development of ex vivo treatment of transplants. We hypothesized that masking MHC ex vivo before transplantation could help to prevent early AMR in presensitized recipients.