Repetitive antigen stimulation in the periphery dictates the composition and recall responses of brain-resident memory CD8 T cells.

The human brain harbors virus-specific, tissue-resident memory (T) CD8 T cells. However, the impact of repeated peripheral viral infection on the generation, phenotype, localization, and recall responses of brain T remains elusive. Here, utilizing two murine models of peripheral viral infection, we demonstrate that circulating memory CD8 T cells with previous antigen exposure exhibit a markedly reduced capacity to form brain T compared to naive CD8 T cells.

Navigated versus conventionally instrumented total knee arthroplasty techniques: No difference in functional alignment or balance.

Purpose: Much debate exists about the superiority of navigated versus conventional instrumentation for achieving optimal balance and alignment during total knee arthroplasty (TKA). Recent registry data indicate no long-term survivorship benefit for TKAs performed using technology assistance, despite the added resource and financial costs. However, outcome comparisons are confounded by varying surgeon techniques and targets for ideal balance and alignment.

Type-I IFNs induce GBPs and lysosomal defense in hepatocytes to control malaria.

parasites undergo development and replication within the hepatocytes before infecting the erythrocytes and initiating clinical malaria. Although type-I interferons (IFNs) are known to hinder infection within the liver, the underlying mechanisms remain unclear. Here, we describe two IFN-I-driven hepatocyte antimicrobial programs controlling liver-stage malaria.

Immunopeptidomics Mapping of Listeria monocytogenes T Cell Epitopes in Mice.

Listeria monocytogenes is a foodborne intracellular bacterial model pathogen. Protective immunity against Listeria depends on an effective CD8 T cell response, but very few T cell epitopes are known in mice as a common animal infection model for listeriosis. To identify epitopes, we screened for Listeria immunopeptides presented in the spleen of infected mice by mass spectrometry-based immunopeptidomics.