Publications by authors named "J Hamuro"
Age-related macular degeneration (AMD) is associated with the dysfunction and degeneration of retinal pigment epithelium (RPE) cells. Here, we examined how the formation and expansions of cell clusters are regulated by the differentiation of the RPE cells. In this study, ARPE-19 cells were cultivated in standard or differentiation media, i.
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- The study investigates whether polishing can remove guttae in Fuchs endothelial corneal dystrophy (FECD) without needing Descemet stripping and if cultured corneal endothelial cell (CEC) transplants can help maintain reduced guttae post-surgery.
- A case series conducted at Kyoto Prefectural University Hospital followed 22 eyes over three years, focusing on changes in guttae after cultured CEC transplant therapy.
- Results showed that all 15 monitored eyes had guttae pre-op, with a notable reduction in guttae presence after surgical intervention, indicating that both procedures can effectively reduce guttae.
Purpose: To reveal the molecular mechanism underlying degeneration in human retinal pigment epithelial (hRPE) cells with dysfunctional mitochondrial homeostasis.
Methods: The expression of recently identified miR-494-3p in extracellular vesicles (EV) released from induced-pluripotential-stem-cell-derived human RPE (iPS-hRPE), during coculture with macrophages (Mps) was investigated in iPS-hRPE and ARPE cells differentiated in the presence of nicotinamide (Nic-ARPE). The expression of phosphatase and tensin homolog (PTEN), sirtuin3 (SIRT3), and mitochondrial marker proteins before and after the transfection of miR-494-3p inhibitor and mimic, and the changes in mitochondrial metabolism, membrane potential, and oxidative phosphorylation (OXPHOS) were monitored.
Article Synopsis
- The study aimed to investigate the relationship between metabolites and microRNAs (miRs) in the aqueous humor of patients with bullous keratopathy (BK) to help preserve corneal endothelial integrity.
- A total of 55 BK patients and 31 cataract patients were analyzed using advanced biostatistical methods like hierarchical clustering and Lasso analysis to assess miR and metabolite levels in their aqueous humor.
- Results indicated that specific metabolites decreased in BK patients, notably 3-hydroxyisobutyric acid, and were positively associated with certain miRs, suggesting a potential mechanism behind the degeneration of human corneal endothelium.
Article Synopsis
- - The study aimed to investigate how extracellular vesicle (EV) microRNAs (miRs) interact to mitigate the degeneration of human corneal endothelium (HCE) tissues.
- - Researchers analyzed miR levels in various corneal tissues and patient samples, identifying distinct groups of miRs that show varying expression based on aging and other factors, particularly highlighting miR-184 and -24-3p for their regenerative potential.
- - Findings revealed that decreasing cellular miR-34a led to increased release of regenerative miRs like miR-184 and -24-3p, suggesting their role in promoting tissue repair and reducing inflammatory responses in corneal cells.