The administration route of indigestible gut permeability markers modulates urinary marker recovery in calves.

Indigestible gut permeability markers are used to assess gut integrity and can be administered to calves via a milk meal (MM) or orally pulsed (OP). This study investigated how marker administration route (ADM_R) affects the estimation of gut permeability in relation to milk replacer (MR) fat inclusion. Thirty-two newborn Holstein calves were blocked based on their arrival sequence at the facility.

Performance, nutrient digestibilities, and metabolic profiles of Holstein calves fed whole milk or milk replacer at two feeding levels.

The macronutrient composition of common milk replacers (MR) largely differs from bovine whole milk (WM). These differences are more nutritionally relevant when calves receive higher milk allowances. Therefore, the present study compared fresh WM with a conventional MR fed at 2 level of feeding allowances evaluating growth performance, apparent total-tract nutrient digestibilities, nitrogen (N) balance, and serum metabolite profiles in calves.

Accurate design of high-affinity protein binding macrocycles using deep learning.

The development of macrocyclic binders to therapeutic proteins typically relies on large-scale screening methods that are resource-intensive and provide little control over binding mode. Despite considerable progress in physics-based methods for peptide design and deep-learning methods for protein design, there are currently no robust approaches for design of protein-binding macrocycles. Here, we introduce RFpeptides, a denoising diffusion-based pipeline for designing macrocyclic peptide binders against protein targets of interest.

'Our culture makes us strong': Understanding and working with community strengths among Aboriginal people in western Sydney.

Background: Strengths-based approaches to health care are often seen as an alternative to deficit-based approaches and are common in Aboriginal health settings. Despite this, there is little existing research that describes Aboriginal peoples' perspectives about the strengths of their communities. This paper describes cultural strengths and resources as understood by Aboriginal people living in western Sydney.

Serum lipidomic profiling of dairy calves fed milk replacers containing animal or vegetable fats.

Vegetable fat blends are commonly used as fat sources in milk replacers (MR) for calves, but their composition differs considerably from that of bovine milk fat. The aim of this study was to investigate the serum lipid profile of preweaning calves fed twice-daily MR containing 30% fat (% DM). Upon arrival, 30 male Holstein-Friesian calves (BW = 45.