Publications by authors named "J H VanDierendonck"
Article Synopsis
- Molecular cloning and gene expression can be difficult when working with toxic proteins, making it challenging to handle such genes in bacteria.
- Researchers created multi-layer control systems that utilize a combination of transcriptional and translational controls, along with reduced plasmid copy numbers, to clone highly toxic proteins effectively.
- The approach includes using weaker promoters with a riboswitch to control toxin expression, enabling the study of effects in living organisms while allowing for controlled overexpression and purification of less toxic proteins.
Reactive oxygen species are used to eradicate malignant cells in photodynamic therapy as well as in other cancer therapies. Despite many efforts, the pathways leading to cellular damage and cell killing due to the action of these species are poorly understood. In previous studies with hematoporphyrin derivative-sensitized L929 murine fibroblasts, the only parameter for which a relation with photodynamically induced reproductive cell death could not be excluded was inhibition of DNA excision repair.
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