Publications by authors named "J H Lorent"
Membranes are molecular interfaces that compartmentalize cells to control the flow of nutrients and information. These functions are facilitated by diverse collections of lipids, nearly all of which are distributed asymmetrically between the two bilayer leaflets. Most models of biomembrane structure and function often include the implicit assumption that these leaflets have similar abundances of phospholipids.
View Article and Find Full Text PDFArticle Synopsis
- - In Gram-negative bacteria, the outer membrane has a unique structure, featuring lipopolysaccharides (LPS) on the outside and glycerophospholipids (GPLs) on the inside, with the Mla system maintaining this balance by removing misplaced GPLs from the outer layer.
- - The study examines how deleting certain components affects the lipid makeup of the bacteria, changing properties like membrane stiffness and antibiotic susceptibility, while noting an overall increase in GPLs and alterations in LPS structure.
- - Understanding these lipid composition changes and their effects on the bacterial membrane can help develop new treatments for infections caused by these bacteria, which are significant in hospital settings.
Microbiota and luminal components may affect epithelial integrity and thus participate in the pathophysiology of colon cancer (CC) and inflammatory bowel disease (IBD). Therefore, we aimed to determine the effects of fecal luminal factors derived from patients with CC and ulcerative colitis (UC) on the colonic epithelium using a standardized colon-derived two-dimensional epithelial monolayer. The complex primary human stem cell-derived intestinal epithelium model, termed RepliGut Planar, was expanded and passaged in a two-dimensional culture which underwent stimulation for 48 h with fecal supernatants (FS) from CC patients ( = 6), UC patients with active disease ( = 6), and healthy subjects (HS) ( = 6).
View Article and Find Full Text PDFOxidative stress in combination with acid stress has been shown to inactivate a wide spectrum of microorganisms, including multi-resistant bacteria. This occurs e.g.
View Article and Find Full Text PDFArticle Synopsis
- - Darier disease (DD) is a rare skin disorder caused by mutations in the ATP2A2 gene, which is essential for maintaining calcium balance in cells.
- - Current research has shown that dantrolene sodium (Dl), a drug that typically treats other conditions, can increase calcium levels in the endoplasmic reticulum and improve cell adhesion in DD cells.
- - The study indicates that Dl could help alleviate key issues related to DD by reducing endoplasmic reticulum stress and preventing cell death, suggesting it might be a viable treatment option for the disease.