An agar medium-based method for screening somatic incompatibility in Agaricus bisporus.

To visualize the nonself recognition reaction in the cultivated mushroom Agaricus bisporus, we developed a method using the azo dye Evans blue. The use of Evans blue highlights dead mycelial sections, which are produced following nonself recognition in the interaction zone between two individuals. This method can differentiate between distinct heterokaryons, as well as between closely related heterokaryons constructed from siblings.

Conversion of Lignocellulosic Biomass Into Valuable Feed for Ruminants Using White Rot Fungi.

White rot fungi can degrade lignin and improve the nutritional value of highly lignified biomass for ruminants. We screened for excellent fungi-biomass combinations by investigating the improvement of digestibility of wheat straw, barley straw, oat straw, rapeseed straw, miscanthus, new reed, spent reed from thatched roofs, and cocoa shells after colonisation by Ceriporiopsis subvermispora (CS), Lentinula edodes (LE), and Pleurotus eryngii (PE) (indicated by increased in vitro gas production [IVGP]). First, growth was evaluated for three fungi on all types of biomass, over a period of 17 days in race tubes.

Impact of local tobacco control policies on the prevalence of smoke-free sports clubs.

Objectives: In absence of national legislation, local governments may stimulate outdoor sports clubs to become smoke-free. However, it is unknown whether and to what extent such efforts are effective in encouraging sports clubs to adopt a smoke-free policy (SFP). The aim of this study was to assess the association between tobacco control policies of municipalities and the prevalence of SFPs among outdoor sports clubs.

From C-lignin to C-mycelium: uses polymeric lignin as a carbon source.

Plant biomass conversion by saprotrophic fungi plays a pivotal role in terrestrial carbon (C) cycling. The general consensus is that fungi metabolize carbohydrates, while lignin is only degraded and mineralized to CO. Recent research, however, demonstrated fungal conversion of C-monoaromatic compounds into proteinogenic amino acids.

Public support for smoke-free private indoor and public outdoor areas in the Netherlands: A trend analysis from 2018-2022.

Introduction: In addition to smoke-free policies in indoor public and workplaces, governments increasingly implement smoke-free policies at beaches, in parks, playgrounds and private cars ('novel smoke-free policies'). An important element in the implementation of such policies is public support. In the context of the ambition of the Netherlands to reach a smoke-free generation by 2040, we investigated temporal changes in public support for novel smoke-free policies.