[Influence of different types of experimental obesity on resistance of the mouse to infection by Salmonella typhimurium and Klebsiella pneumoniae].

Resistance to infections inducing two types of immune response, humoral and cell-mediated, has been measured in mice after Salmonella typhimurium and Klebsiella pneumoniae inoculation; the animals exhibited different kinds of obesity: genetic, ob/ob and db/db mutants, induced by fat diet or gold thioglucose (aurothioglucose) injection (determining obesity of central origin). Klebsiella infection was aggravated in all types of obesity. Salmonella infection was aggravated in genetically diabetic and dietary-obese mice.

[Preparation of immunosorbents from lipopolysaccharides and polysaccharides extracted from various gram-negative and gram-positive bacteria].

The method of binding of lipopolysaccharides (LPS) extracted from Salmonella typhimurium to aminohexyl-sepharose 4B by activation with benzoquinone was applied to three different LPS extracted from several enterobacteria species: S. seftenberg 1,3,19, S. cholerae suis 6(2),7 and Escherichia coli O141:H32.

[Preparation of monospecific anti-Salmonella LPS antibodies from bacteria immobilized in polyacrylamide gel].

The use of an immunoabsorbent obtained by entrapping Salmonella cells into a polyacrylamide gel lattice enabled us to obtain anti-O monospecific immune sera which can be used for a quick serological identification of some species of Salmonella in the course of a diagnosis. In this paper we describe a method for immobilization of S. typhimurium as well as the preparation of monospecific anti-05 antibodies from plurispecific anti-S.

Preparation of monospecific anti-Salmonella lipopolysaccharide antibodies by affinity chromatography.

The use of immunoadsorbent obtained by coupling aminohexyl-sepharose 4B with Salmonella lipopolysaccharide (LPS) by means of benzoquinone enabled us to obtain anti-O monospecific immune sera which can be used for a quick serological identification of some species of Salmonella in the course of a diagnosis. In this paper we describe a method for binding the LPS extracted from S. typhi-murium with aminohexyl-sepharose 4B, insoluble matrix as well as the preparation of monospecific anti-O5 antibodies from plurispecific anti-S.

[Cellular response in the mouse to two antigenic extracts from "Vibrio cholerae" (author's transl)].

At the cellular level the primary response in Balb/c mice to an antigen prepared from a strain of Vibrio cholerae Ogawa was studied by a localized vibriolytic plaque procedures which permits the enumeration of immunocytes. The antibody response to a single injection with identical doses of antigen was comparatively studied by both intraperitoneal and subcutaneous immunization. The highest titre was noted two weeks after the intraperitoneal and three days after the subcutaneous injection.