Use of short, wide-bore capillary columns in GC toxicological screening.

The usefulness of wide-bore, thick film capillary columns for routine toxicological screening was assessed. Two such columns were examined: fused silica CPSil 5CB (i.d.

Rapid determination of ethylene glycol in biological material.

A rapid and simple modification of a gas chromatographic (AFID) method of ethylene glycol determination, based on derivation with n-butylboronic acid, was developed.

Impact of biological matrix, drug concentration, and method of isolation on detectability and variability of retention index values in gas chromatography.

The retention indices of eight basic and neutral drugs extracted from plasma, blood, or liver homogenate by six different methods were calculated using temperature-programmed gas chromatography on OV-1 and SE-30 columns. Two concentrations were applied: 1 and 50 mg/L, respectively. The type of extraction had great influence on recovery and analytical background.

Enzymic hydrolysis of tissues before XAD-2 extraction in poisoning cases.

Samples of blood, liver and brain taken from 13 cases of fatal poisoning with barbiturates, antidepressants, phenothiazines and anticonvulsants were digested with trypsin and beta-glucuronidase. The concentrations of drugs were higher in hydrolyzed livers in barbiturate and phenothiazine poisonings. In the case of diazepam, phenytoin, carbamazepine, amitriptyline, imipramine and chloroprothixene poisonings the results obtained after enzymic hydrolysis were the same or worse than in controls.