Squamous cell carcinoma of the subglottis.

Study Design: A retrospective study over a 25-year period of patients with laryngeal carcinoma treated by the Department of Otolaryngology at Wake Forest University.

Methods: The boundaries of the subglottis were defined as 5 mm below the free edge of the true vocal folds extending to the inferior border of the cricoid cartilage. All were staged according to American Joint Committee on Cancer: stages I and II were considered early and stages III and IV as late.

Sister chromatid exchanges in lymphocytes of patients with oral carcinoma.

Sister chromatid exchanges (SCEs) were studied in cultured peripheral lymphocytes of 22 untreated patients with squamous cell carcinoma of the oral cavity and 29 age- and sex-matched controls. The SCE rate in cancer patients was not significantly higher compared with that found in controls, but there was a significant correlation between the SCE rate in lymphocytes of the cancer patients and the size of the primary tumor.

The reliability of frozen-section diagnosis in various breast lesions: a study based on 3451 biopsies.

In this study, we analyze breast biopsy material over a 5-year period and we compare the results of frozen-section diagnosis with the corresponding diagnosis of the permanent histologic sections. In this study, which represents the largest series in the available international literature, it is proven that frozen-section diagnosis of various breast lesions is a highly accurate procedure in spite of the difficulties facing the pathologist with the frequent borderline or premalignant lesions. The great responsibility of the pathologist is emphasized.

Lymphocyte reactivity in patients with cancer of the oral cavity measured by sister chromatid differential staining.

Lymphocyte reactivity in phytohemagglutinin-stimulated peripheral lymphocyte cultures from 22 patients with squamous cell carcinoma of the oral cavity and 29 age- and sex-matched controls was measured by the bromodeoxyuridine-Giemsa method for demonstrating sister chromatid differential staining. One hundred metaphases from each donor were counted, and the proportions of cells in the first, second, and third cycles were scored to estimate the in vitro mitogen responsiveness and cell division rates of the lymphocytes. The in vitro reactivity of peripheral lymphocytes of cancer patients was impaired in comparison with that of the controls, and the degree of reactivity of lymphocytes in cancer patients was not related to the size (greatest diameter) of the primary tumor.

Karyotypic abnormalities of squamous cell carcinoma of the oral cavity.

The karyotypic abnormalities in 18 squamous cell carcinomas of the oral cavity were studied in unbanded chromosomes on direct preparations of the tumor material. The chromosome counts revealed a great variability in the number of chromosomes per cell of each tumor, the range being from 31 to 148 in all cases studied. The modal population of cells was diploid in five cases, triploid in eight cases, tetraploid and pentaploid in one case each.