Preparation of Nanocomposite Biopolymer Films from Willd Starch and Their Nanostructures as a Potential Replacement for Single-Use Polymers.

This study explored the effect of incorporating cellulose and starch nanoparticles, obtained from the Willd plant, on the physical and chemical properties of starch-based films derived from the same plant. Additionally, the synergistic effect of combining the nanostructures was assessed. The nanocomposite biopolymer films were prepared by the casting method using 1 and 3 wt% concentrations of the nanostructures (CNCs: cellulose nanocrystals, CNFs: cellulose nanofibers, SNCs: starch nanocrystals), or their blend.

The splicing machinery is dysregulated and represents a therapeutic vulnerability in breast cancer.

Breast cancer (BCa) is a highly prevalent pathological condition (̴30% in women) with limited and subtype-dependent prognosis and therapeutic options. Therefore, BCa management might benefit from the identification of novel molecular elements with clinical potential. Since splicing process is gaining a great relevance in cancer, this work analysed the expression of multiple Spliceosome Components (SCs = 17) and Splicing Factors (SFs = 26) and found a drastic dysregulation in BCa (n = 69) vs.

Generation of an inducible dCas9-SAM human PSC line for endogenous gene activation.

The CRISPR/Cas9 system has transformed genome editing by enabling precise modifications for diverse applications. Recent advancements, including base editing and prime editing, have expanded its utility beyond conventional gene knock-out and knock-in strategies. Additionally, several catalytically dead Cas9 (dCas9) proteins fused to distinct activation domains have been developed to modulate endogenous gene expression when directed to their regulatory regions by specific single-guide RNAs.

Challenges in developing response evaluation criteria for peptide receptor radionuclide therapy: A consensus report from the European Neuroendocrine Tumor Society Advisory Board Meeting 2022 and the ENETS Theranostics Task Force.

Assessing the response to systemic therapy in neuroendocrine tumors (NET) is challenging since morphological imaging response is often delayed and not necessarily reflective of clinical benefit. Peptide receptor radionuclide therapy (PRRT) has a complex mechanism of action, further complicating response assessment. In response to these challenges, the European Neuroendocrine Tumor Society (ENETS) Theranostics Task Force conducted a statement-based survey among experts to identify the current landscape and unmet needs in PRRT response assessment.

Optimization of protein identifications through the use of different chromatographic approaches and bioinformatic pipelines.

Rationale: Selection of proteomic workflows for a given project can be a daunting task. This research provides a guide outlining the impact on protein identification of different steps such as chromatographic separation, data acquisition strategies, and bioinformatic pipelines. The data presented here will help experts and nonexpert proteomic users to increase proteome coverage and peptide identification.